Details

Autor(en) / Beteiligte

• Yu, Qi
• Zhang, Kenneth Yin
• Liang, Hua
• Zhao, Qiang
• Yang, Tianshe
• Liu, Shujuan
• Zhang, Chuanqi
• Shi, Zhengjian
• Xu, Wenjuan
• Huang, Wei

Titel

Dual-Emissive Nanohybrid for Ratiometric Luminescence and Lifetime Imaging of Intracellular Hydrogen Sulfide

Ist Teil von

• ACS applied materials & interfaces, 2015-03, Vol.7 (9), p.5462-5470

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• We design a nanohybrid for the detection of hydrogen sulfide (H2S) based on mesoporous silica nanoparticles (MSNs). A phosphorescent iridium­(III) complex and a specific H2S-sensitive merocyanine derivative are embedded into the nanohybrid. It exhibits a unique dual emission that is ascribed to the iridium­(III) complex and the merocyanine derivative, respectively. Upon addition of sodium hydrogen sulfide (NaHS), the emission from the merocyanine derivative is quenched, while the emission from the iridium­(III) complex is almost unchanged, which enables the ratiometric detection of H2S. Additionally, the nanohybrid has a long luminescence lifetime and displays a significant change in luminescence lifetime in response to H2S. Intracellular detection of H2S is performed via ratiometric imaging and photoluminescence lifetime imaging microscopy. Compared with the intensity-based method, the lifetime-based detection is independent of the probe concentration and can efficiently distinguish the signals of the probe from the autofluorescence in complex biological samples.