Details

Autor(en) / Beteiligte

• Özer Yücel, Özlem
• Berker, Ezel
• Mesci, Lütfiye
• Eratalay, Kenan
• Tepe, Eser
• Tezcan, İlhan

Titel

Analysis of TNF-α (-308) polymorphism and gingival crevicular fluid TNF-α levels in aggressive and chronic periodontitis: A preliminary report

Ist Teil von

• Cytokine (Philadelphia, Pa.), 2015-04, Vol.72 (2), p.173-177

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• •The putative role of TNF-α (-308) genotype in periodontal disease outcome was investigated.•Correlation between TNF-α (-308) genotype, and GCF TNF-α levels was also analyzed.•TNF-α (-308) allele 2 frequency may be associated with aggressive periodontitis.•TNF-α may aggravate rapid destruction of periodontal attachment and bone loss in aggressive periodontitis. The purpose of this study was to determine the differences in the distribution of TNF-α (-308) gene polymorphism among aggressive periodontitis, chronic periodontitis and periodontally healthy individuals and also to investigate whether this polymorphism is associated with gingival crevicular fluid TNF-α levels and periodontal disease severity. A total of 93 individuals were enrolled in the study including 38 aggressive periodontitis, 29 chronic periodontitis patients, and 26 healthy controls. Single nucleotide polymorphism at TNF-α (-308) is analyzed by PCR-RFLP method. Gingival crevicular fluid samples were analyzed for TNF-α, using ELISA. The distribution of genotypes and allele frequencies for TNF-α (-308) were similar among the groups. After stratification of patients with respect to attachment level, aggressive periodontitis patients with clinical attachment level ⩾4mm was observed to have a higher frequency of TNF-α (-308) allele 2 compared to the chronic periodontitis patients with clinical attachment level ⩾4mm. No significant differences were found between the TNF-α levels of the different genotypes in spite of an insignificant increase in patient groups carrying TNF-α (-308) allele 2. The results of this study revealed an association between TNF-α (-308) allele 2 frequency and aggressive periodontitis patients with clinical attachment level ⩾4mm in the population studied.