Details

Autor(en) / Beteiligte

• Ganas, Carolin
• Weiß, Annika
• Nazarenus, Moritz
• Rösler, Susanne
• Kissel, Thomas
• Rivera_Gil, Pilar
• Parak, Wolfgang J.

Titel

Biodegradable capsules as non-viral vectors for in vitro delivery of PEI/siRNA polyplexes for efficient gene silencing

Ist Teil von

• Journal of controlled release, 2014-12, Vol.196, p.132-138

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• The efficiency of siRNA delivery is demonstrated to be improved by encapsulating the siRNA within a non-viral carrier based on layer-by-layer assembly of oppositely charged biodegradable and biocompatible polyelectrolytes. In comparison to other non-viral delivery vehicles such as polycation-based complexes, a smaller amount of siRNA was necessary to produce in vitro gene silencing as early as 20–30h after incubation. Colloidal carriers based on assembled biodegradable polyelectrolytes offer several advantages, such as efficient intracellular delivery after endocytosis followed by release to the cytosol, as well as protection of the siRNA, which is crucial for its therapeutic activity. [Display omitted]