Yeast (Chichester, England), 1993-04, Vol.9 (4), p.351-361
1993
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Details
- Autor(en) / Beteiligte
- Titel
- Molecular genetics in Saccharomyces kluyveri: The HIS3 homolog and its use as a selectable marker gene in S. kluyveri and Saccharomyces cerevisiae
- Ist Teil von
- Yeast (Chichester, England), 1993-04, Vol.9 (4), p.351-361
- Ort / Verlag
- Chichester, UK: John Wiley & Sons, Ltd
- Erscheinungsjahr
- 1993
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We cloned the Saccharomyces kluyveri HIS3 homolog, k‐HIS3, and made a partial deletion of the gene. The k‐HIS3 gene complemented a HIS3 deletion in S. cerevisiae. The DNA sequences of the open reading frames (ORFs) of the HIS3 homologs are 70% identical at the DNA level and 83% identical at the deduced amino acid level. The ORF upstream of the k‐HIS3 gene is related to the PET56 gene of S. cerevisiae found upstream of the HIS3 gene of S. cerevisiae. The ORF downstream from the k‐HIS3 gene is not related to the DED1 gene found downstream of the HIS3 gene in S. cerevisiae.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0749-503XeISSN: 1097-0061DOI: 10.1002/yea.320090405Titel-ID: cdi_proquest_miscellaneous_16602897
- Format
- –
- Schlagworte
- Amino Acid Sequence, Base Sequence, Biological and medical sciences, Chromosome Mapping, Cloning, Molecular, Fundamental and applied biological sciences. Psychology, Genes, Fungal - genetics, Genes. Genome, Genetic Markers, HIS3, Hydro-Lyases - genetics, Molecular and cellular biology, Molecular genetics, Molecular Sequence Data, Mutagenesis, Saccharomyces - genetics, Saccharomyces cerevisiae, Saccharomyces cerevisiae - genetics, Saccharomyces kluyveri, Selection, Genetic, Sequence Analysis, DNA, Transformation, Genetic, Uracil - metabolism