Details

Autor(en) / Beteiligte

• Zavec, Apolonija Bedina
• Ota, Ajda
• Zupancic, Tina
• Komel, Radovan
• Ulrih, Nataša Poklar
• Liovic, Mirjana

Titel

Archaeosomes can efficiently deliver different types of cargo into epithelial cells grown in vitro

Ist Teil von

• Journal of biotechnology, 2014-12, Vol.192, p.130-135

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• •We tested a type of lyposomes (archaeosomes) derived from polar lipids of Aeropyrum pernix K1 on keratinocytes and SW13 cells in vitro.•Our unilamellar archaeosomes were used as means of delivery for small molecules, smaller proteins, large protein aggregates and plasmid DNA.•Our archaeosomes are not toxic to keratinocytes even at high doses.•Our archaeosomes may be used as basis to develop a universal system for therapy delivery to epithelial tissues, in particular skin. Archaeosomes are a type of liposomes prepared from the polar lipids of various Archaeobacteria. These have unique structural features that increase the lipid bilayer's stability even under high temperatures, low or high pH, presence of phospholipases and bile salts. This makes them ideal as basis for the development of new drug, gene and vaccine delivery systems. In this study we prepared large unilamellar archaeosomes (400nm size) from Aeropyrum pernix K1 and demonstrated their potential as base for the development of an efficient and universal system for drug or therapy delivery to epithelial cells. Our archaeosomes may be used to deliver small fluorescent molecules (calcein), smaller proteins (60kDa listeriolysin), large protein aggregates (e.g. keratin 14) and plasmid DNA, into epithelial cells grown in culture. The delivery efficiency for small molecules is already quite high at this initial stage of development, around 40%. Our unilamellar archaeosomes are also not toxic to keratinocytes even at high doses (500μg/ml).