Neuroscience research, 2015-02, Vol.91, p.1-7
2015
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- Autor(en) / Beteiligte
- Titel
- BDNF promoter I methylation correlates between post-mortem human peripheral and brain tissues
- Ist Teil von
- Neuroscience research, 2015-02, Vol.91, p.1-7
- Ort / Verlag
- Ireland: Elsevier Ireland Ltd
- Erscheinungsjahr
- 2015
- Quelle
- Access via ScienceDirect (Elsevier)
- Beschreibungen/Notizen
- •The periphery correlates with the brain in term of BDNF promoter I methylation levels.•The periphery could be used to reflect the brain for methylation in BDNF promoter I.•BDNF promoters I and IV are correlated in term of methylation in the hippocampus.•Males showed higher BDNF promoter I methylation levels compared to females in brain, blood and muscle tissues. Several psychiatric disorders have been associated with CpG methylation changes in CG rich promoters of the brain-derived neurotrophic factor (BDNF) mainly by extracting DNA from peripheral blood cells. Whether changes in peripheral DNA methylation can be used as a proxy for brain-specific alterations remains an open question. In this study we aimed to compare DNA methylation levels in BDNF promoter regions in human blood cells, muscle and brain regions using bisulfite-pyrosequencing. We found a significant correlation between the levels of BDNF promoter I methylation measured in quadriceps and vPFC tissues extracted from the same individuals (n=98, Pearson, r=0.48, p=4.5×10−7). In the hippocampus, BDNF promoter I and IV methylation levels were strongly correlated (Pearson, n=37, r=0.74, p=1.4×10−7). We found evidence for sex-dependent effect on BDNF promoter methylation levels in the various tissues and blood samples. Taken together, these data indicate a strong intra-individual correlation between peripheral and brain tissue. They also suggest that sex determines methylation patterns in BDNF promoter region across different types of tissue, including muscle, brain, and blood.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0168-0102eISSN: 1872-8111DOI: 10.1016/j.neures.2014.10.003Titel-ID: cdi_proquest_miscellaneous_1658708404
- Format
- –
- Schlagworte
- Autopsy, BDNF, Bipolar Disorder - genetics, Bipolar Disorder - metabolism, Blood Cells - metabolism, Brain - metabolism, Brain-Derived Neurotrophic Factor - blood, Brain-Derived Neurotrophic Factor - genetics, Brain-Derived Neurotrophic Factor - metabolism, DNA Methylation, Epigenetics, Female, Humans, Male, Methylation, Muscle, Skeletal - metabolism, Organ Specificity, Prefrontal cortex, Promoter, Promoter Regions, Genetic, Quadriceps, Sex Factors