Details

Autor(en) / Beteiligte

• Badura, Aleksandra
• Schonewille, Martijn
• Voges, Kai
• Galliano, Elisa
• Renier, Nicolas
• Gao, Zhenyu
• Witter, Laurens
• Hoebeek, Freek E.
• Chédotal, Alain
• De Zeeuw, Chris I.

Titel

Climbing Fiber Input Shapes Reciprocity of Purkinje Cell Firing

Ist Teil von

• Neuron (Cambridge, Mass.), 2013-05, Vol.78 (4), p.700-713

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2013

Quelle

MEDLINE

Beschreibungen/Notizen

• The cerebellum fine-tunes motor activity via its Purkinje cell output. Purkinje cells produce two different types of spikes, complex spikes and simple spikes, which often show reciprocal activity: a periodical increase in complex spikes is associated with a decrease in simple spikes, and vice versa. This reciprocal firing is thought to be essential for coordinated motor behavior, yet how it is accomplished is debated. Here, we show in Ptf1a::cre;Robo3lox/lox mice that selectively rerouting the climbing fibers from a contralateral to an ipsilateral projection reversed the complex-spike modulation during sensory stimulation. Strikingly, modulation of simple spikes, which is supposed to be controlled by mossy fibers, reversed as well. Climbing fibers enforce this reciprocity in part by influencing activity of inhibitory interneurons, because the phase of their activity was also converted. Ptf1a::cre;Robo3lox/lox mice showed severe ataxia highlighting that climbing fiber input and its impact on reciprocity of Purkinje cell firing play an important role in motor coordination. •Climbing fiber input dominates reciprocity of Purkinje cell firing•Complex-spike activity in Purkinje cells can override simple-spike firing patterns•Climbing fiber input contributes to modulation of molecular layer interneurons•Misrouting of climbing fiber pathway results in ataxia The cerebellum fine-tunes motor activity via Purkinje cell output. Here, Badura et al. show that reciprocity of Purkinje cell complex and simple spike firing is shaped by climbing fiber activity. Disrupting reciprocity results in ataxia, highlighting the importance of climbing fiber input in motor coordination.