Details

Autor(en) / Beteiligte

• Gasparotto, Juliana Machado
• Werle, Leandro Bernardi
• Foletto, Edson L
• Kuhn, Raquel C
• Jahn, Sergio Luiz
• Mazutti, Marcio A

Titel

Production of Cellulolytic Enzymes and Application of Crude Enzymatic Extract for Saccharification of Lignocellulosic Biomass

Ist Teil von

• Applied biochemistry and biotechnology, 2015-01, Vol.175 (1), p.560-572

Ort / Verlag

Boston: Springer-Verlag

Erscheinungsjahr

2015

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• In this study, the optimal conditions for production of cellulolytic enzymes by Trichoderma reesei NRRL-6156 using the solid-state fermentation were assessed in conical flasks and validated in a packed-bed bioreactor. Afterwards, the crude enzymatic extract obtained in the optimized condition was used for hydrolysis of sugarcane bagasse in water and ultrasound baths. The enzyme activities determined in this work were filter paper, exocellulase, endocellulase, and xylanase. The optimized condition for production was moisture content 68.6 wt% and soybean bran concentration 0.9 wt%. The crude enzymatic extract was applied for hydrolysis of sugarcane bagasse, being obtained 224.0 and 229 g kg⁻¹at temperature of 43.4 °C and concentration of enzymatic extract of 18.6 % in water and ultrasound baths, respectively. The yields obtained are comparable to commercial enzymes.