Details

Autor(en) / Beteiligte

• Hewage, Ranuka T.
• Aree, Thammarat
• Mahidol, Chulabhorn
• Ruchirawat, Somsak
• Kittakoop, Prasat

Titel

One strain-many compounds (OSMAC) method for production of polyketides, azaphilones, and an isochromanone using the endophytic fungus Dothideomycete sp

Ist Teil von

• Phytochemistry (Oxford), 2014-12, Vol.108, p.87-94

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Polyketides (1–3), an isochromanone 4, and azaphilone 5 and 6 are produced by one strain-many compounds (OSMAC) approach. Metabolite production of the fungus Dothideomycete sp. depends on culture media and source of potato and malt extracts used for the preparation of media. [Display omitted] •Fungal metabolites were produced by one strain-many compounds (OSMAC) approach.•An endophytic fungus Dothideomycete sp. was a metabolite producer for the OSMAC method.•Metabolite production of the fungus Dothideomycete sp. depends on culture media.•Slight alteration of media composition enables the fungus changes metabolite composition. Polyketides 1–6 were produced by a one strain-many compounds (OSMAC) approach using the endophytic fungus Dothideomycete sp. CRI7 as a producer. Metabolite production of the fungus Dothideomycete sp. CRI7 was sensitive to sources of potato and malt extract used for the preparation of PDB and Czapek malt media, respectively. Three hitherto unknown metabolites were obtained from the fungus CRI7 grown in PDB medium prepared from a commercial potato powder instead of fresh tubers of potato, while three others were obtained from the fungus cultivated in Czapek malt medium. Moreover, a source of malt extract used in the Czapek malt medium was found to influence metabolite production by the fungus CRI7. Structure elucidation of these compounds was achieved by analysis of spectroscopic data, as well as by single crystal X-ray analysis. Two of the compounds showed weak cytotoxic activity, while the remainders were inactive toward the cell lines tested. One compound exhibited radical scavenging activity with an IC50 value of 21.7μM, and inhibited aromatase with an IC50 value of 12.3μM.