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Development of a Biological Protocol for Endotoxin Detection Using Quartz Crystal Microbalance (QCM)
Ist Teil von
Applied biochemistry and biotechnology, 2014-12, Vol.174 (7), p.2492-2503
Ort / Verlag
Boston: Springer-Verlag
Erscheinungsjahr
2014
Link zum Volltext
Quelle
SpringerLink
Beschreibungen/Notizen
In this paper, a biological protocol for endotoxin detection has been developed and optimized by quartz crystal microbalance (QCM). The parameters involved in the formation of the self-assembled monolayer (SAM) have been analyzed, and a study of the pH of the ligand buffer has been performed in order to find the best condition for the ligand immobilization and, in consequence, for the endotoxin detection. The detection limit obtained with the characterized biological protocol corresponds to 1.90 μg/ml. The effectiveness of the optimized biological protocol has been analyzed by cyclic voltammetry analysis.