Details

Autor(en) / Beteiligte

• Soares, Sónia
• Amaral, Joana S.
• Oliveira, Maria Beatriz P.P.
• Mafra, Isabel

Titel

Improving DNA isolation from honey for the botanical origin identification

Ist Teil von

• Food control, 2015-02, Vol.48, p.130-136

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2015

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Honey is a natural product highly consumed due its known association with health benefits. Monofloral honeys are perceived as better quality products, being the most appreciated by consumers, thus attaining higher market values. Therefore efficient tools are needed as alternatives to the classical microscopic analysis presently used for the botanical origin identification of honey. In the present work, the use of DNA-based methods for the botanical species identification of honey is proposed. For this purpose, five DNA extraction methods (the kits NucleoSpin Plant (methods A and B) and DNeasy Plant Mini Kit, and the in-house CTAB-based and Wizard methods) combined with three different sample pre-treatments were applied to four honey samples (3 monofloral honeys of Calluna vulgaris, Lavandula spp. and Eucalyptus spp. and one multifloral honey). The 15 DNA extraction protocols were compared in terms of DNA integrity, yield and purity, as well as capacity of amplification targeting universal and adh1 specific genes of C. vulgaris. The results demonstrated the superior efficacy of the Wizard method in terms of DNA quality and amplification capacity, when combined with the sample preparation treatment with a mechanical disruption step of pollen to improve DNA yield. Although with considerable lower DNA yields, the CTAB and DNeasy methods were also successful because both were able to clearly amplify heather DNA from the monofloral heather honey. •DNA-based methods were combined with sample pre-treatments applied to honey.•The 15 DNA extraction protocols applied to 4 honey samples were compared.•Results of DNA integrity, yield, purity and amplification capacity were compared.•PCR amplification was carried out targeting universal and adh1 heather specific genes.•The Wizard method exhibited the best performance for DNA quality and amplification.