Biomaterials, 2014-08, Vol.35 (25), p.6810-6821
2014
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- Autor(en) / Beteiligte
- Titel
- Regulation of human endothelial progenitor cell maturation by polyurethane nanocomposites
- Ist Teil von
- Biomaterials, 2014-08, Vol.35 (25), p.6810-6821
- Ort / Verlag
- Netherlands: Elsevier Ltd
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract The mobilization and homing of endothelial progenitor cells (EPCs) are critical to the development of an antithrombotic cardiovascular prosthesis. Polyurethane (PU) with superior elasticity may provide a mechanical environment resembling that of the natural vascular tissues. The topographical cues of PU were maximized by making nanocomposites with a small amount of gold nanoparticles (AuNPs). The nanocomposites of PU-AuNPs (“PU-Au”) with a favorable response of endothelial cells were previously established. In the current study, the effect of PU and PU-Au nanocomposites on the behavior of human peripheral blood EPCs was investigated in vitro and in vivo. It was found that PU-Au promoted EPCs to become differentiated endothelial cells in vitro, confirmed by the increased expressions of CD31 and VEGF-R2 surface markers. The increased maturation of EPCs was significantly more remarkable on PU-Au, probably through the stromal derived factor 1α (SDF-1α)/CXCR4 signaling pathway. In vivo experiments showed that EPCs seeded on PU-Au coated catheters effectively reduced thrombosis by differentiation into endothelial cells. Surface endothelialization with CD31 and CD34 expression as well as intimal formation with α-SMA expression was significantly accelerated in the group receiving EPC-seeded PU-Au catheters. Moreover, the analysis of collagen deposition revealed a reduction of fibrosis in the group receiving EPC-seeded PU-Au catheters as compared to the other groups. These results suggest that EPCs engineered with a proper elastic substrate may provide unique endothelialization and antithrombogenic properties that benefit vascular tissue regeneration.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0142-9612eISSN: 1878-5905DOI: 10.1016/j.biomaterials.2014.04.076Titel-ID: cdi_proquest_miscellaneous_1642230401
- Format
- –
- Schlagworte
- Advanced Basic Science, Animals, Biocompatibility, Biomedical materials, Catheters, Cell Differentiation - drug effects, Cell Proliferation - drug effects, Cells, Cultured, Chemokine CXCL12 - genetics, Chemokine CXCL12 - metabolism, Dentistry, Endothelial cells, Endothelial progenitor cells (EPCs), Endothelial Progenitor Cells - drug effects, Endothelial Progenitor Cells - metabolism, Endothelium, Vascular - cytology, Endothelium, Vascular - drug effects, Female, Fibrinolytic Agents - chemistry, Fibrinolytic Agents - pharmacology, Gold - chemistry, Gold nanoparticles (AuNPs), Humans, Leukocytes, Mononuclear - drug effects, Leukocytes, Mononuclear - metabolism, Male, Maturation, Metal Nanoparticles - chemistry, Nanocomposites, Nanocomposites - chemistry, Nitric Oxide Synthase Type II - genetics, Nitric Oxide Synthase Type II - metabolism, Plutonium, Polyurethane, Polyurethanes - chemistry, Polyurethanes - pharmacology, Rabbits, Receptors, CXCR4 - antagonists & inhibitors, Receptors, CXCR4 - genetics, Receptors, CXCR4 - metabolism, Signal Transduction, Surface Properties, Surgical implants, Vascular grafts