Details

Autor(en) / Beteiligte

• Miao, Yanming
• Zhang, Zhifeng
• Gong, Yan
• Yan, Guiqin

Titel

Phosphorescent quantum dots/doxorubicin nanohybrids based on photoinduced electron transfer for detection of DNA

Ist Teil von

• Biosensors & bioelectronics, 2014-09, Vol.59, p.300-306

Ort / Verlag

Kidlington: Elsevier B.V

Erscheinungsjahr

2014

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• MPA-capped Mn-doped ZnS QDs/DXR nanohybrids (MPA: 3-mercaptopropionic acid; QDs: quantum dots; DXR: cetyltrimethyl ammonium bromide) were constructed via photoinduced electron transfer (PIET) and then used as a room-temperature phosphorescence (RTP) probe for detection of DNA. DXR as a quencher will quench the RTP of Mn-doped ZnS QDs via PIET, thereby forming Mn-doped ZnS QDs/DXR nanohybrids and storing RTP. With the addition of DNA, it will be inserted into DXR and thus DXR will be competitively desorbed from the surface of Mn-doped ZnS QDs, thereby releasing the RTP of Mn-doped ZnS QDs. Based on this, a new method for DNA detection was built. The sensor for DNA has a detection limit of 0.039mgL−1 and a linear range from 0.1 to 14mgL−1. The present QDs-based RTP method does not need deoxidants or other inducers as required by conventional RTP detection methods, and avoids interference from autofluorescence and the scattering light of the matrix that are encountered in spectrofluorometry. Therefore, this method can be used to detect the DNA content in body fluid. •MPA-capped Mn-doped ZnS QDs and DXR are combined through photoinduced electron transfer to form Mn-doped ZnS quantum dots/DXR nanohybrids and thus to quench the RTP intensity of Mn-doped ZnS QDs.•This method improves the DNA detection ability of QDs and provides an important method for developing more convenient and effective DNA detection sensors.•The interferences from autofluorescence and scattering light were avoided. The developed biosensor does not need any complicated sample pretreatment. This method can be used to detect the content of DNA in body fluid.