Details

Autor(en) / Beteiligte

• Russ, Brendan E.
• Olshanksy, Moshe
• Smallwood, Heather S.
• Li, Jasmine
• Denton, Alice E.
• Prier, Julia E.
• Stock, Angus T.
• Croom, Hayley A.
• Cullen, Jolie G.
• Nguyen, Michelle L.T.
• Rowe, Stephanie
• Olson, Matthew R.
• Finkelstein, David B.
• Kelso, Anne
• Thomas, Paul G.
• Speed, Terry P.
• Rao, Sudha
• Turner, Stephen J.

Titel

Distinct Epigenetic Signatures Delineate Transcriptional Programs during Virus-Specific CD8+ T Cell Differentiation

Ist Teil von

• Immunity (Cambridge, Mass.), 2014-11, Vol.41 (5), p.853-865

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• The molecular mechanisms that regulate the rapid transcriptional changes that occur during cytotoxic T lymphocyte (CTL) proliferation and differentiation in response to infection are poorly understood. We have utilized ChIP-seq to assess histone H3 methylation dynamics within naive, effector, and memory virus-specific T cells isolated directly ex vivo after influenza A virus infection. Our results show that within naive T cells, codeposition of the permissive H3K4me3 and repressive H3K27me3 modifications is a signature of gene loci associated with gene transcription, replication, and cellular differentiation. Upon differentiation into effector and/or memory CTLs, the majority of these gene loci lose repressive H3K27me3 while retaining the permissive H3K4me3 modification. In contrast, immune-related effector gene promoters within naive T cells lacked the permissive H3K4me3 modification, with acquisition of this modification occurring upon differentiation into effector/memory CTLs. Thus, coordinate transcriptional regulation of CTL genes with related functions is achieved via distinct epigenetic mechanisms. [Display omitted] •Specific H3K4me3 and H3K27me3 patterns identify functionally related CTL genes•Many CTL-specific transcription factors are bivalent for H3K4me3 and H3K27me3•Bivalent loci largely resolve to a permissive signature rapidly after activation•H3K4me2 identifies a subset of rapidly transcribed immune-specific gene loci It is unclear how changes in genome-wide histone methylation status regulate distinct transcriptional signatures observed in naive, effector, and memory virus-specific cytotoxic lymphocytes. Russ et al. identify key epigenetic signatures that delineate distinct transcriptional programs that underpin CD8+ T cell differentiation during infection.