Details

Autor(en) / Beteiligte

• Taskinen, Barbara
• Zauner, Dominik
• Lehtonen, Soili I
• Koskinen, Masi
• Thomson, Chloe
• Kähkönen, Niklas
• Kukkurainen, Sampo
• Määttä, Juha A. E
• Ihalainen, Teemu O
• Kulomaa, Markku S
• Gruber, Hermann J
• Hytönen, Vesa P

Titel

Switchavidin: Reversible Biotin–Avidin–Biotin Bridges with High Affinity and Specificity

Ist Teil von

• Bioconjugate chemistry, 2014-12, Vol.25 (12), p.2233-2243

Ort / Verlag

United States: American Chemical Society

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Switchavidin is a chicken avidin mutant displaying reversible binding to biotin, an improved binding affinity toward conjugated biotin, and low nonspecific binding due to reduced surface charge. These properties make switchavidin an optimal tool in biosensor applications for the reversible immobilization of biotinylated proteins on biotinylated sensor surfaces. Furthermore, switchavidin opens novel possibilities for patterning, purification, and labeling.