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Toward the harmonization of result presentation for the eosin‐5′‐maleimide binding test in the diagnosis of hereditary spherocytosis
Cytometry. Part B, Clinical cytometry, 2015-01, Vol.88 (1), p.50-57
Hunt, Linda
Greenwood, David
Heimpel, Hermann
Noel, Nigel
Whiteway, Alastair
King, May‐Jean
2015
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Hunt, Linda
Greenwood, David
Heimpel, Hermann
Noel, Nigel
Whiteway, Alastair
King, May‐Jean
Titel
Toward the harmonization of result presentation for the eosin‐5′‐maleimide binding test in the diagnosis of hereditary spherocytosis
Ist Teil von
Cytometry. Part B, Clinical cytometry, 2015-01, Vol.88 (1), p.50-57
Ort / Verlag
United States: Wiley Subscription Services, Inc
Erscheinungsjahr
2015
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
Background The eosin‐5′‐maleimide (EMA) Binding test measures reduced mean channel fluorescence (MCF) reading of EMA‐labeled red cells (EMA‐RBCs) from patients with hereditary spherocytosis (HS). Reporting test results can be either in the actual MCF reading or as a ratio by normalization of the test MCF result to the mean MCF value of six normal controls. The latter format has potential for universal reporting. Methods We analyzed three years' archival MCF data from HS and non‐HS patient groups for establishment of reference ranges of ratios for normal adults and HS. A prospective study used FC500 and FACS Canto II cytometers to analyze contemporaneously EMA‐RBCs from several patient groups and normal donors. Statistical analyses of the prospective data determined the cut‐off values, and the sensitivity and specificity for HS respectively for the MCF and the ratio result presentations. The effect of using fewer than six normal controls for the ratio denominator was explored. Results The FC500 gave a mean ratio of 0.782 (SD = 0.086) in HS patients with an optimal cut‐off ratio of 0.918 (98.7% specificity, 95.6% sensitivity), and gray area ratio of 0.868–0.918. The Canto II gave a mean ratio of 0.774 (SD = 0.085) with an optimal cut‐off ratio of 0.925 (97.1% specificity and 100% sensitivity), and gray area ratio of 0.859–0.925. Conclusion Harmonization of result presentation is feasible with no apparent constraint by instrument design. Interpretation of gray‐area data requires an assessment of patient's clinical presentation and family history or performing a family study. © 2014 International Clinical Cytometry Society
Sprache
Englisch
Identifikatoren
ISSN: 1552-4949
eISSN: 1552-4957
DOI: 10.1002/cyto.b.21187
Titel-ID: cdi_proquest_miscellaneous_1637574136
Format
–
Schlagworte
Adolescent
,
Adult
,
Ankyrins - deficiency
,
Child
,
Child, Preschool
,
EMA binding test
,
Eosine Yellowish-(YS) - analogs & derivatives
,
Erythrocytes - chemistry
,
Erythrocytes - pathology
,
Female
,
flow cytometry
,
Flow Cytometry - standards
,
Fluorescent Dyes
,
harmonization of EMA test
,
hereditary spherocytosis
,
Humans
,
Infant
,
Infant, Newborn
,
Male
,
Middle Aged
,
Reference Values
,
Sensitivity and Specificity
,
Spherocytosis, Hereditary - diagnosis
,
Spherocytosis, Hereditary - pathology
,
Staining and Labeling - standards
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