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Effect of natamycin on cytochrome P450 enzymes in rats
Ist Teil von
Food and chemical toxicology, 2013-12, Vol.62, p.281-284
Ort / Verlag
Oxford: Elsevier Ltd
Erscheinungsjahr
2013
Link zum Volltext
Quelle
MEDLINE
Beschreibungen/Notizen
[Display omitted]
•The effect of natamycin on cytochrome P450 (CYP) enzyme activities in rat liver microsomes were determined in vivo.•Male Wistar rats were treated orally with natamycin at doses of 0.3, 1, 3 and 10mg/kgbw/day for 6 consecutive days.•Natamycin (1, 3 and 10mg/kgbw) significantly decreased CYP2E1, CYP1A1/2, CYP2B1/2 and CYP4A1/2 enzyme activities.•These new data can contribute to understand the mode of action of the toxicity of natamycin.
Natamycin is a polyene macrolide antibiotic widely used in the food industry as a feed additive to prevent mold contamination of foods. There are many contradictory results on the genotoxic effects of macrolides which could suggest a potential risk for humans. In the present study, the effects of natamycin on the activities of some drug metabolizing enzymes in rat liver microsomes were determined in vivo. Rats were treated orally with natamycin at doses of 0.3, 1, 3 and 10mg/kg body weight (bw)/day for 6days. Determinations of cytochrome P450 (CYP) enzyme activities were carried out in hepatic microsomes isolated from rats treated. The activities of CYP2E1, CYP1A1/2 CYP2B1/2 and CYP4A1/2 enzymes significantly decreased after treatment with 1, 3 and 10mg/kgbw/day, in a dose-dependent manner as compared to control. This effect was not observed after natamycin treatment at dose of 0.3mg/kgbw/day. Our results suggest that natamycin may not potentiate the toxicity of many xenobiotics via metabolic activation and/or accumulation of reactive metabolites but also might affect the clearance of other xenobiotics detoxified by the studied CYP enzymes.