Details

Autor(en) / Beteiligte

• MOZZI, F
• REBULLA, P
• LLILLO, F
• VARNIER, O. E
• BIADATI, C
• CALCAGNO, L
• MELOTTI, S
• SIRCHIA, G

Titel

HIV and HTLV infections in 1305 transfusion-dependent thalassemics in Italy

Ist Teil von

• AIDS (London), 1992-05, Vol.6 (5), p.505-508

Ort / Verlag

Hagerstown, MD: Lippincott Williams & Wilkins

Erscheinungsjahr

1992

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• To evaluate the prevalence of antibodies to HIV-1/2 and HTLV-I/II in 1305 transfusion-dependent square -thalassemics treated in 36 centres in Italy. Sera were screened using an enzyme-linked immunosorbent assay (ELISA) containing viral lysate antigens from HIV-1 and HIV-2, and a particle agglutination assay for the detection of antibodies to HTLV-I and HTLV-II. Repeatedly reactive samples were examined by Western Blot (WB) assays containing recombinant and viral lysate antigens. Differential diagnosis was finally made by ELISA based on synthetic peptides. The residual risk of HIV-1 infection through blood transfusion after the implementation of anti-HIV-1 screening in blood donors in Italy was approximately 1:50,000 blood units; this is based on an approximate number of 200,000 blood units administered to our group of patients during 1986-1990 and the occurrence of four new anti-HIV-1 seroconversions.