The Journal of biological chemistry, 1997-11, Vol.272 (47), p.29729-29734
1997
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Details
- Autor(en) / Beteiligte
- Titel
- Notch-1 Controls the Expression of Fatty Acid-activated Transcription Factors and Is Required for Adipogenesis
- Ist Teil von
- The Journal of biological chemistry, 1997-11, Vol.272 (47), p.29729-29734
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 1997
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Notch, a transmembrane receptor member of the homeotic epidermal growth factor-like family of proteins, participates in cell-to-cell signaling to control cell fate during development. Activated Notch-1 constructs lacking the extracellular region prevent differentiation of several mammalian cells in vitro. This effect, however, bypasses the normal mechanisms of cell-to-cell interactions in which Notch-1 participates. We investigated the role of Notch-1 in the hormone-induced adipocyte differentiation of 3T3-L1 fibroblasts, a paradigmatic model of adipogenesis that requires cell-to-cell contact. Unlike other differentiation models, Notch-1 expression and function were necessary conditions for adipogenesis. Impaired Notch-1 expression by antisenseNotch-1 constructs prevented adipocyte differentiation. Strategies aimed at blocking putative Notch/ligand interactions also blocked adipogenesis, implicating Notch as a critical molecule in cell-to-cell signaling necessary for differentiation. Inhibition of Notch-1 expression or function decreased the expression of peroxisomal proliferator-activated receptors δ and γ, transcription factors that control adipocyte differentiation and that are up-regulated at cell confluence. These results implicate Notch in the commitment of 3T3-L1 cells to undergo adipogenesis by controlling the expression of the principal regulators of this process.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0021-9258eISSN: 1083-351XDOI: 10.1074/jbc.272.47.29729Titel-ID: cdi_proquest_miscellaneous_16310138
- Format
- –
- Schlagworte
- 3T3 Cells, Adipocytes - cytology, Adipocytes - drug effects, Adipose Tissue - cytology, Adipose Tissue - growth & development, ADIPOSE TISSUES, ADN, Animals, ANTISENSE DNA, ARN MENSAJERO, ARN MESSAGER, Cell Communication, CELL DIFFERENTIATION, CELLS, CELLULE, CELULAS, CONTROL HORMONAL, CONTROLE HORMONAL, DIFERENCIACION CELULAR, DIFFERENCIATION CELLULAIRE, DNA, EXPRESION GENICA, EXPRESSION DES GENES, FACTEUR DE TRANSCRIPTION, FACTORES DE TRANSCRIPCION, Fatty Acids - metabolism, FIBROBLASTE, FIBROBLASTOS, FIBROBLASTS, GENE EXPRESSION, GENETIC TRANSFORMATION, HORMONAL CONTROL, INHIBICION, INHIBITION, INSULIN, Insulin - pharmacology, INSULINA, INSULINE, Membrane Proteins - physiology, MESSENGER RNA, Mice, Oligonucleotides, Antisense - metabolism, PEROXISOMAL PROLIFERATOR ACTIVATED RECEPTORS, RAT, RATA, RATS, Receptor, Notch1, RECEPTORS, Receptors, Cell Surface, Receptors, Cytoplasmic and Nuclear - metabolism, Recombinant Proteins - pharmacology, TEJIDO ADIPOSO, TISSU ADIPEUX, TRANSCRIPTION FACTORS, Transcription Factors - biosynthesis, Transcription Factors - metabolism, Transfection, TRANSFORMACION GENETICA, TRANSFORMATION GENETIQUE