Details

Autor(en) / Beteiligte

• Abreu, Marcus da Matta, MD, PhD
• Pazetti, Rogerio, PhD
• Almeida, Francine Maria de, MSc
• Correia, Aristides Tadeu, BSc
• Parra, Edwin Roger, MD, PhD
• Silva, Laís Pereira da
• Vieira, Rodolfo de Paula, PhD
• Pêgo-Fernandes, Paulo Manuel, MD, PhD
• Jatene, Fabio Biscegli, MD, PhD

Titel

Methylene blue attenuates ischemia–reperfusion injury in lung transplantation

Ist Teil von

• The Journal of surgical research, 2014-12, Vol.192 (2), p.635-641

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

Access via ScienceDirect (Elsevier)

Beschreibungen/Notizen

• Abstract Background Ischemia–reperfusion injury (IRI) is one of the principal obstacles for the lung transplantation (LTx) success. Several strategies have been adopted to minimize the effects of IRI in lungs, including ex vivo conditioning of the grafts and the use of antioxidant drugs, such as methylene blue (MB). We hypothesized that MB could minimize the effects of IRI in a LTx rodent model. Methods Forty rats were divided into four groups ( n  = 10) according to treatment (saline solution or MB) and graft cold ischemic time (3 or 6 h). All animals underwent unilateral LTx. Recipients received 2 mL of saline or MB intraperitoneally before transplantation. After 2 h of reperfusion, arterial blood and exhaled nitric oxide samples were collected and bronchoalveolar lavage performed. Then animals were euthanized, and histopathology analysis as well as cell counts and cytokine levels measurements in bronchoalveolar lavage fluid were performed. Results There was a significant decrease in exhaled nitric oxide, neutrophils, interleukin-6, and tumor necrosis factor-α in MB-treated animals. PaO2 and uric acid levels were higher in MB group. Conclusions MB was able in attenuating IRI in this LTx model.