Oncology reports, 2015-01, Vol.33 (1), p.201-206
2015
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- Autor(en) / Beteiligte
- Titel
- DJ-1 knockdown inhibits growth and xenograft-induced tumor generation of human hepatocellular carcinoma cells
- Ist Teil von
- Oncology reports, 2015-01, Vol.33 (1), p.201-206
- Ort / Verlag
- Greece: D.A. Spandidos
- Erscheinungsjahr
- 2015
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- The aim of this study was to identify potential downstream effectors of the oncogene DJ-1 in hepatocellular carcinoma (HCC) cell lines, and examine its role in the Akt signaling pathway and HCC oncogenesis. Expression of the DJ-1 protein was assessed by immunoblotting in several human HCC cell lines. Knockdown of DJ-1 was achieved by transfecting DJ-1-specific short hairpin RNAs into the HepG2 HCC cell line. The effect of DJ-1 downregulation on phosphatase and tensin homolog (PTEN) and phosphorylated Akt was evaluated. In addition, cell cycle, proliferation, adhesion and invasion were analyzed in the DJ-1 knockdown of HepG2 cells. The growth of HepG2-induced tumor was evaluated in a nude mouse model after DJ-1 silencing. Stable DJ-1 knockdown was achieved in HepG2 cells using a shRNA eukaryotic expression vector. Downregulation of DJ-1 increased PTEN expression but decreased phosphorylation of Akt in HepG2 cells. In addition, DJ-1 knockdown resulted in the decreased proliferation, adhesion and invasion of HepG2 cells in vitro, and inhibited the growth of HepG2-induced tumor in vivo. DJ-1 knockdown altered the malignant behavior of HepG2 cells, potentially through the Akt signaling pathway, suggesting a crucial role for DJ-1 in the oncogenesis of HCC.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1021-335XeISSN: 1791-2431DOI: 10.3892/or.2014.3594Titel-ID: cdi_proquest_miscellaneous_1628241202
- Format
- –
- Schlagworte
- Akt signaling pathways, Analysis, Animals, Apoptosis, Carcinoma, Hepatocellular - metabolism, Carcinoma, Hepatocellular - pathology, Cell Adhesion, Cell culture, Cell growth, Cell Movement, Cell Proliferation, Cellular signal transduction, Development and progression, Diagnosis, DJ-1, Gene Knockdown Techniques, HCC, Hep G2 Cells, Hepatitis, Hepatoma, HepG2, Humans, Immunoglobulins, Intracellular Signaling Peptides and Proteins - genetics, Intracellular Signaling Peptides and Proteins - metabolism, Kinases, Liver cancer, Liver Neoplasms - metabolism, Liver Neoplasms - pathology, Male, Medical prognosis, Metastasis, Mice, Inbred BALB C, Mice, Nude, Oncogene Proteins - genetics, Oncogene Proteins - metabolism, Phosphorylation, Plasmids, Protein Deglycase DJ-1, Protein Processing, Post-Translational, Proteins, Proto-Oncogene Proteins c-akt - metabolism, PTEN Phosphohydrolase - metabolism, Risk factors, RNA, Small Interfering - genetics, Tumorigenesis