FEBS letters, 2014-08, Vol.588 (17), p.3202-3212
2014
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- Autor(en) / Beteiligte
- Titel
- Calcium signaling mediates the response to cadmium toxicity in Saccharomyces cerevisiae cells
- Ist Teil von
- FEBS letters, 2014-08, Vol.588 (17), p.3202-3212
- Ort / Verlag
- England: Elsevier B.V
- Erscheinungsjahr
- 2014
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- •High environmental Cd2+ induces Ca2+ cytosolic bursts in yeast cells.•Mn2+, Co2+, Ni2+, Zn2+, or Hg2+ do not induce Ca2+-mediated signaling.•Yeast tolerance to Cd2+ correlates with Ca2+ transport and homeostasis. The involvement of Ca2+ in the response to high Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+, and Hg2+ was investigated in Saccharomyces cerevisiae. The yeast cells responded through a sharp increase in cytosolic Ca2+ when exposed to Cd2+, and to a lesser extent to Cu2+, but not to Mn2+, Co2+, Ni2+, Zn2+, or Hg2+. The response to high Cd2+ depended mainly on external Ca2+ (transported through the Cch1p/Mid1p channel) but also on vacuolar Ca2+ (released into the cytosol through the Yvc1p channel). The adaptation to high Cd2+ was influenced by perturbations in Ca2+ homeostasis. Thus, the tolerance to Cd2+ often correlated with sharp Cd2+-induced cytosolic Ca2+ pulses, while the Cd2+ sensitivity was accompanied by the incapacity to rapidly restore the low cytosolic Ca2+.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0014-5793eISSN: 1873-3468DOI: 10.1016/j.febslet.2014.07.001Titel-ID: cdi_proquest_miscellaneous_1627967572
- Format
- –
- Schlagworte
- 2-(N-morpholino)ethanesulfonic acid, [Ca2+]cyt, Aequorin, Biological Transport - drug effects, Cadmium, Cadmium - metabolism, Cadmium - toxicity, Calcium - metabolism, Calcium signaling, Calcium Signaling - drug effects, Cation Transport Proteins - metabolism, Cell Membrane - drug effects, Cell Membrane - metabolism, cytAEQ, Cytosol - drug effects, Cytosol - metabolism, cytosolic aequorin, cytosolic Ca2, divalent cation of any of the metals tested, Dose-Response Relationship, Drug, endoplasmic reticulum, Environmental Pollutants - metabolism, Environmental Pollutants - toxicity, Heavy metal stress, Homeostasis - drug effects, Iron-Binding Proteins - metabolism, Me2, MES, Mutation, Oxidative Stress - drug effects, parental “wild type” cells, relative luminescence units, RLU, Saccharomyces cerevisiae, Saccharomyces cerevisiae - cytology, Saccharomyces cerevisiae - drug effects, Saccharomyces cerevisiae - genetics, Saccharomyces cerevisiae - metabolism, Saccharomyces cerevisiae Proteins - metabolism, standard error, synthetic dextrose medium, Transcription Factors - metabolism, yeast extract-peptone-dextrose medium, YPD