Details

Autor(en) / Beteiligte

• Barrios, C
• Lussow, A R
• Van Embden, J
• Van der Zee, R
• Rappuoli, R
• Costantino, P
• Louis, JA
• Lambert, P-H
• Del Giudice, G

Titel

Mycobacterial heat-shock proteins as a carrier molecules. II: The use of the 70kDa mycobacterial heat-shock protein as carrier for conjugated vaccines can circumvent the need for adjuvants and Bacillus Calmette Guerin priming

Ist Teil von

• European journal of immunology, 1992-01, Vol.22 (6), p.1365-1372

Erscheinungsjahr

1992

Link zum Volltext

Quelle

Wiley Online Library

Beschreibungen/Notizen

• In a recent work, we have shown that mycobacterial heat-shock proteins (hsp) of 65-kDa (GroEL-type) and 70-kDa (DnaK-type) acted as carrier molecules in mice, previously primed with Mycobacterium tuberculosis var. bovis (Bacillus Calmette-Guerin, BCG), for the induction of high and long-lasting titers of IgG against the repetitive malaria synthetic peptide (NANP) sub(40). Anti-peptide antibodies were induced when the malaria peptide, conjugated to the mycobacterial hsp, was given in the absence of any adjuvants. In this report, we show that mice immunized with peptides or oligosaccharides conjugated to the 70-kDa hsp produced high titers of IgG antibodies in the absence of any previous priming with BCG. The findings strongly suggest that the use of hsp as carriers in conjugated constructs for the induction of anti-peptide and anti-oligosaccharide antibodies could be of value in the design of new vaccines for eventual use in humans.