Details

Autor(en) / Beteiligte

• Ishikawa, Aki
• Yoto, Yuko
• Tsugawa, Takeshi
• Tsutsumi, Hiroyuki

Titel

Quantitation of human parvovirus B19 DNA in erythema infectiosum and aplastic crisis

Ist Teil von

• Journal of medical virology, 2014-12, Vol.86 (12), p.2102-2106

Ort / Verlag

United States: Blackwell Publishing Ltd

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• Several publications concerning the methods of real‐time PCR for human parvovirus B19 (B19V) have appeared and some case reports mention B19V DNA loads. However, no large‐scale study quantitating levels of B19V DNA in common or representative B19V manifestations such as erythema infectiosum and aplastic crisis has been performed. Consequently, using the TaqMan PCR assay, the B19V load in a large sample of subjects with erythema infectiosum or aplastic crisis was quantitated. Sixty‐five subjects in the acute phase of erythema infectiosum were involved, and in addition 22 serum samples from seven subjects with B19V‐associated aplastic crisis complicating chronic hemolytic anemia were also analyzed. In the acute phase of erythema infectiosum the median B19V DNA load in the serum samples from the acute phase of erythema infectiosum was 7.63 × 105 genomes/ml, (range from 4.48 × 103 to 8.31 × 106 genomes/ml). The serum B19V DNA load during the acute phase of aplastic crisis complicating chronic hemolytic anemia was extremely high, that is 1010–1013 genomes/ml, and decreased gradually to around 105 genomes/ml over 1–2 months. Although all subjects followed an almost uniform and typical clinical course of erythema infectiosum, there was a large individual variation of B19V DNA loads, that is differences of over 1,000 times. Extremely high B19V loads were observed in subjects with aplastic crisis. This study is the first large scale report of studies of the B19V DNA loads in subjects with erythema infectiosum and aplastic crisis, the most common and significant clinical manifestations by B19V infections. J. Med. Virol. 86:2102–2106, 2014. © 2014 Wiley Periodicals, Inc.