Journal of experimental botany, 2014-10, Vol.65 (19), p.5557-5566
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Reassimilation of ammonium in Lotus japonicus
- Ist Teil von
- Journal of experimental botany, 2014-10, Vol.65 (19), p.5557-5566
- Ort / Verlag
- England: Oxford University Press [etc.]
- Erscheinungsjahr
- 2014
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- This review summarizes the most recent results obtained in the analysis of two important metabolic pathways involved in the release of internal sources of ammonium in the model legume Lotus japonicus: photorespiratory metabolism and asparagine breakdown mediated by aparaginase (NSE). The use of photorespiratory mutants deficient in plastidic glutamine synthetase (GS2) enabled us to investigate the transcriptomics and metabolomic changes associated with photorespiratory ammonium accumulation in this plant. The results obtained indicate the existence of a coordinate regulation of genes involved in photorespiratory metabolism. Other types of evidence illustrate the multiple interconnections existing among the photorespiratory pathway and other processes such as intermediate metabolism, nodule function, and secondary metabolism in this plant, all of which are substantially affected in GS2-deficient mutants because of the impairment of the photorespiratory cycle. Finally, the importance of asparagine metabolism in L. japonicus is highlighted because of the fact that asparagine constitutes the vast majority of the reduced nitrogen translocated between different organs of this plant. The different types of NSE enzymes and genes which are present in L. japonicus are described. There is a particular focus on the most abundant K+-dependent LjNSE1 isoform and how TILLING mutants were used to demonstrate by reverse genetics the importance of this particular isoform in plant growth and seed production. This paper analyses the metabolic consequences of photorespiratory ammonium accumulation in Lotus japonicus using omics approaches and highlights the crucial importance of asparaginase in nitrogen remobilization in this plant.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-0957eISSN: 1460-2431DOI: 10.1093/jxb/eru260Titel-ID: cdi_proquest_miscellaneous_1622594350
- Format
- –
- Schlagworte
- Amino acid metabolism, Ammonium Compounds - metabolism, asparaginase, Asparagine - metabolism, Biological Transport, Carbohydrate metabolism, Cell Respiration, Energy metabolism, Enzymes, Gene Expression Regulation, Plant, Genes, Glutamate-Ammonia Ligase - genetics, Glutamate-Ammonia Ligase - metabolism, Legumes, Light, Loteae - genetics, Loteae - metabolism, Loteae - radiation effects, Lotus corniculatus var. japonicus, Molecular Structure, Mutation, Nitrogen, Nitrogen - metabolism, Photorespiration, Plastids - enzymology, Potassium - metabolism, Quaternary ammonium compounds, Reverse Genetics, REVIEW PAPER, Seeds - genetics, Seeds - metabolism, Seeds - radiation effects, Transcriptome