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The use of natural nitrogen isotopes to detect animal waste-produced nitrate in streams was demonstrated with a minimal number of samples and minimal supporting data. A second-order stream adjacent to a research dairy farm near Raleigh, NC, had mean nitrate-N levels of 0.4 mg/l with mean super(15)N-nitrate values of 8.7% downstream of the farm. Secondary dairy lagoon liquids used for spray irrigation had a mean ammonium concentration of 87.5 mg/l and a mean super(15)N level of 20.7%. A small, intermittent first-order stream draining dairy sprayfields and emptying into the second-order stream had a mean nitrate-N level of 3.7 mg/l and mean super(15)N-nitrate of 16%. The observed increase in super(15)N values with increasing nitrate level in this stream indicates that a small amount of waste-produced nitrate is being exported.