Molecular medicine reports, 2014-12, Vol.10 (6), p.2967-2972
2014
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Details
- Autor(en) / Beteiligte
- Titel
- LY294002 suppresses the malignant phenotype and sensitizes osteosarcoma cells to pirarubicin chemotherapy
- Ist Teil von
- Molecular medicine reports, 2014-12, Vol.10 (6), p.2967-2972
- Ort / Verlag
- Greece: D.A. Spandidos
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Pirarubicin is frequently used in chemotherapy against tumors. However, clinical use is severely limited by the development of progressive dose-dependent cardiomyopathy and acquired drug resistance. LY294002 is a commonly used pharmacologic inhibitor, which selectively inhibits the phosphoinositide 3-kinase-AKT nexus. The aim of this study was to investigate the combined inhibitory effect of LY294002 and pirarubicin on human osteosarcoma (OS) cells in vitro. The inhibitory effect of LY294002 plus pirarubicin on U2-OS and MG-63 OS cell proliferation, apoptosis, migration and invasion was investigated by cell proliferation, wound healing and Transwell invasion assays. The results revealed that LY294002 and pirarubicin synergistically induced apoptosis, and inhibited the growth, migration and invasion of OS cells. This indicates that LY294002 enhanced the effects of pirarubicin on OS in vitro. LY294002 combined with pirarubicin may thus be a future therapeutic strategy in OS.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1791-2997eISSN: 1791-3004DOI: 10.3892/mmr.2014.2617Titel-ID: cdi_proquest_miscellaneous_1615744973
- Format
- –
- Schlagworte
- AKT protein, Antineoplastic Agents - pharmacology, Apoptosis, Apoptosis - drug effects, Binding sites, Bone cancer, Bone Neoplasms - drug therapy, Bone Neoplasms - metabolism, Bone Neoplasms - pathology, Cancer therapies, Cardiomyopathy, Cell cycle, Cell growth, Cell Line, Tumor, Cell Movement - drug effects, Cell proliferation, Cell Proliferation - drug effects, Chemotherapy, Chromones - pharmacology, Doxorubicin - analogs & derivatives, Doxorubicin - pharmacology, Drug dosages, Drug resistance, Drug therapy, Genetic aspects, Humans, Kinases, LY294002, metastasis, Methods, Morpholines - pharmacology, Osteosarcoma, Osteosarcoma - drug therapy, Osteosarcoma - metabolism, Osteosarcoma - pathology, Osteosarcoma cells, Phenotype, Phosphatidylinositol 3-Kinases - metabolism, Physiological aspects, pirarubicin, Proteins, Proto-Oncogene Proteins c-akt - metabolism, Sarcoma, Studies, synergistic action, Tumors, Wound healing