Developmental biology, 2014-11, Vol.395 (1), p.154-166
2014
Details
- Autor(en) / Beteiligte
- Titel
- FGFR-ERK signaling is an essential component of tissue separation
- Ist Teil von
- Developmental biology, 2014-11, Vol.395 (1), p.154-166
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2014
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Formation of a constriction and tissue separation between parent and young polyp is a hallmark of the Hydra budding process and controlled by fibroblast growth factor receptor (FGFR) signaling. Appearance of a cluster of cells positive for double phosphorylated ERK (dpERK) at the late separation site indicated that the RAS/MEK/ERK pathway might be a downstream target of the Hydra Kringelchen FGFR. In fact, inhibition of ERK phosphorylation by the MEK inhibitor U0126 reversibly delayed bud detachment and prevented formation of the dpERK-positive cell cluster indicating de novo-phosphorylation of ERK at the late bud base. In functional studies, a dominant-negative Kringelchen FGFR prevented bud detachment as well as appearance of the dpERK-positive cell cluster. Ectopic expression of full length Kringelchen, on the other hand, induced a localized rearrangement of the actin cytoskeleton at sites of constriction, localized ERK-phosphorylation and autotomy of the body column. Our data suggest a model in which (i) the Hydra FGFR targets, via an unknown pathway, the actin cytoskeleton to induce a constriction and (ii) FGFR activates MEK/ERK signaling at the late separation site to allow tissue separation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0012-1606eISSN: 1095-564XDOI: 10.1016/j.ydbio.2014.08.010Titel-ID: cdi_proquest_miscellaneous_1609306676
- Format
- –
- Schlagworte
- Animals, Animals, Genetically Modified, Blotting, Western, Butadienes - pharmacology, Enzyme Inhibitors - pharmacology, Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors, Extracellular Signal-Regulated MAP Kinases - metabolism, Gene Expression Regulation, Developmental, Green Fluorescent Proteins - genetics, Green Fluorescent Proteins - metabolism, Hydra - embryology, Hydra - growth & development, Hydra - metabolism, In Situ Hybridization, Microscopy, Confocal, Microscopy, Fluorescence, Morphogenesis - drug effects, Morphogenesis - genetics, Nitriles - pharmacology, Phosphorylation - drug effects, Pyrroles - pharmacology, Receptors, Fibroblast Growth Factor - antagonists & inhibitors, Receptors, Fibroblast Growth Factor - metabolism, Signal Transduction, Time Factors