Details

Autor(en) / Beteiligte

• Green, Douglas R.
• Bissonnette, Reid
• Zheng, Heguang
• Onda, Takebumi
• Echeverri, Fernando
• Mogil, Rona J.
• Steele, J. Kevin
• Voralia, Michael
• Fotedar, Arun

Titel

Immunoregulatory Activity of the T-Cell Receptor α Chain Demonstrated by Retroviral Gene Transfer

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 1991-10, Vol.88 (19), p.8475-8479

Ort / Verlag

Washington, DC: National Academy of Sciences of the United States of America

Erscheinungsjahr

1991

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• We have previously described an antigen-specific I-Ad-restricted T-cell hybridoma, A1.1, that constitutively releases an antigen-specific immunoregulatory activity into supernatants. Using retrovirally mediated gene transfer, we have found that transfer of the T-cell receptor α chain (TCRα) gene from A1.1 to a number of other T-cell hybridomas effectively transferred the ability to produce the activity. Gene transfer of the TCR β chain (TCRβ), however, did not transfer this ability. The regulatory activity from cells expressing the A1.1 TCRα bound to and was eluted from an anti-TCRα monoclonal antibody and displayed fine antigenic specificity identical to that of supernatants from A1.1. The possibility that this activity represents a secreted form of the TCRα (as opposed to shed cell-surface TCR) was examined in BW1100 cells, lacking TCRα and TCRβ, which produced the antigen-specific activity after gene transfer of the A1.1 TCRα gene. The expression of the immunoregulatory activity in supernatants correlated with a direct antigen-binding activity as detected by ELISA, thus raising the possibility that antigen binding is relevant to the mechanism of action of the soluble TCRα. We discuss these observations and our earlier studies suggesting an immunoregulatory role for soluble TCRα.