Details

Autor(en) / Beteiligte

• Macip, Salvador
• Mezquita, Cristóbal
• Mezquita, Jovita

Titel

Alternative transcriptional initiation and alternative use of polyadenylation signals in the αB-crystallin gene expressed in different chicken tissues

Ist Teil von

• Gene, 1997-03, Vol.187 (2), p.253-257

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

1997

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Overexpression of αB-crystallin is associated with numerous neurodegenerative diseases and abnormal cell growth patterns. To study the mechanisms involved in the control of the transcriptional activity of the gene we have characterized its expression in different chicken tissues. The sequence of the αB-crystallin cDNA isolated from chicken testis and 6-day-old chick embryo is highly homologous to the duck αB-crystallin cDNA and differs from the previously reported chicken lens αB-crystallin cDNA in the 5′ untranslated region (5′- UTR) and in one amino acid of the coding sequence. Four forms of the αB-crystallin cDNA detected in chicken testes arise from the use of alternative transcription initiation sites and alternative polyadenylation signals. The two principal hybridizing bands found in lens and embryonic tissues possess a short 5′- UTR and differ in the length of the 3′- UTR. Forms with longer 5′- UTR are present in testis, muscle, and heart. The use of different start sites and polyadenylation signals could modulate transcriptional activity and the stability of the messages. The expression of the αB-crystallin gene decreases from day 6 to day 8 of chick embryogenesis, in parallel with the expression of the polyubiquitin gene UbII.