Details

Autor(en) / Beteiligte

• Leisy, Douglas J.
• Mattson, Jeanine D.
• Quistad, Gary B.
• Kramer, Steven J.
• Van Beek, Nikolai
• Tsai, Leslie W.
• Enderlin, Frances E.
• Woodworth, Alison R.
• Digan, Mary Ellen

Titel

Molecular cloning and sequencing of cDNAs encoding insecticidal peptides from the primitive hunting spider, Plectreurys tristis (Simon)

Ist Teil von

• Insect biochemistry and molecular biology, 1996-05, Vol.26 (5), p.411-417

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

1996

Quelle

Elsevier Journal Backfiles on ScienceDirect (DFG Nationallizenzen)

Beschreibungen/Notizen

• Plectreurys tristis cephalothorax mRNA was isolated and amplified by PCR using degenerate primers corresponding to reverse translated mature Plt-VI toxin. An oligonucleotide corresponding to a portion of the amplified product was then used to screen a P. tristis cDNA library. The cDNAs from 10 positive clones were sequenced. Eight of these cDNAs corresponded to Plt-VI toxin, one to Plt-XI toxin, and one was very similar to Plt-VIII toxin, with the exception of a single amino acid substitution. Analysis of these cDNAs indicated that these toxins are initially synthesized as prepro-forms which undergo signal cleavage followed by additional processing at both their N- and C-termini to produce the mature products.