Nature genetics, 1996-12, Vol.14 (4), p.421-429
1996
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Details
- Autor(en) / Beteiligte
- Titel
- A PCR-based approach for isolating pathogen resistance genes from potato with potential for wide application in plants
- Ist Teil von
- Nature genetics, 1996-12, Vol.14 (4), p.421-429
- Ort / Verlag
- London: Nature Publishing Group
- Erscheinungsjahr
- 1996
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Plant genes for pathogen resistance can be used to engineer disease resistant crops. Oligonucleotides were designed from sequence motifs conserved between resistance genes of tobacco and Arabidopsis thaliana and used as PCR primers in potato DNA. Amplification products were obtained that were homologous to known resistance genes and linked without recombination with the nematode resistance locus Gro1 and the Phytophthora infestans resistance locus R7 of potato. Map positions of PCR-derived potato gene fragments were also correlated with resistance loci of the related tomato and tobacco genomes. Our results indicate that plant resistance genes that are effective against nematodes, fungi, viruses and bacteria may be isolated based on common sequence motifs and PCR methodology.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1061-4036eISSN: 1546-1718DOI: 10.1038/ng1296-421Titel-ID: cdi_proquest_miscellaneous_15827113
- Format
- –
- Schlagworte
- ADN, Amino Acid Sequence, AMINO ACID SEQUENCES, Arabidopsis - genetics, ARABIDOPSIS THALIANA, Biological and medical sciences, BIOLOGICAL DIFFERENCES, CARTE GENETIQUE, CHEMICAL COMPOSITION, Chromosome Mapping, CHROMOSOME MAPS, Classical genetics, quantitative genetics, hybrids, COMPOSICION QUIMICA, COMPOSITION CHIMIQUE, Conserved Sequence, DIFERENCIAS BIOLOGICAS, DIFFERENCE BIOLOGIQUE, DISEASE RESISTANCE, DNA, ENFERMEDADES DE LAS PLANTAS, Fundamental and applied biological sciences. Psychology, genbank/u60069, genbank/u60070, genbank/u60071, genbank/u60072, genbank/u60073, genbank/u60074, genbank/u60075, genbank/u60076, genbank/u60077, genbank/u60078, genbank/u60079, genbank/u60080, genbank/u60081, genbank/u60082, genbank/u60083, genbank/u60084, GENE, GENES, Genes, Plant, GENETIC MAPPING, GENETIC MAPS, GENETICA, GENETICS, Genetics of eukaryotes. Biological and molecular evolution, GENETIQUE, ISOLATION, LINKAGE GROUPS, LOCI, LOCUS, Lycopersicon esculentum, MALADIE DES PLANTES, MAPAS GENETICOS, Molecular Sequence Data, MULTIGENE FAMILIES, Nicotiana, NICOTIANA TABACUM, NUCLEOTIDE SEQUENCE, PCR, PEPTIDE, PEPTIDES, PEPTIDOS, PESTS OF PLANTS, Phytophthora infestans, PLAGAS DE PLANTAS, PLANT DISEASES, Plant Diseases - genetics, Plants - genetics, Plants - microbiology, Plants - parasitology, Plants, Toxic, POLYMERASE CHAIN REACTION, Polymerase Chain Reaction - methods, Polymorphism, Restriction Fragment Length, POLYPEPTIDES, Pteridophyta, spermatophyta, RAVAGEUR DES PLANTES, RESISTANCE AUX MALADIES, RESISTENCIA A LA ENFERMEDAD, RFLP, SECUENCIA NUCLEOTIDICA, SEQUENCE NUCLEOTIDIQUE, SOLANUM TUBEROSUM, Solanum tuberosum - genetics, SPECIES DIFFERENCES, STRUCTURAL GENES, Vegetals