Proceedings of the National Academy of Sciences - PNAS, 1990-04, Vol.87 (8), p.2901-2905
1990
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Details
- Autor(en) / Beteiligte
- Titel
- Genes that can be mutated to unmask hidden antigenic determinants in the cuticle of the nematode Caenorhabditis elegans
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1990-04, Vol.87 (8), p.2901-2905
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1990
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Rabbit antisera directed against a mixture of proteins solubilized from the wild-type adult Caenorhabditis elegans cuticle were used to isolate mutants, induced by ethyl methanesulfonate treatment, that exhibit alterations in surface antigenicity by immunofluorescence. Genetic mapping and complementation data for four such mutations define two genes, srf-2(I) and srf-3(IV). The mutant phenotypes observed by immunofluorescence appear to result from unmasking of antigenic determinants that are normally hidden in the wild-type cuticle. In support of this hypothesis, surface radioiodination experiments indicate that components labeled on the wild-type surface are missing or less readily labeled on the surface of srf-2 and srf-3 mutants.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.87.8.2901Titel-ID: cdi_proquest_miscellaneous_15774844
- Format
- –
- Schlagworte
- Animals, Antibodies, ANTIGENE, ANTIGENOS, ANTIGENS, Antigens, Helminth - analysis, Antigens, Helminth - genetics, Antigens, Surface - analysis, Antigens, Surface - genetics, Antiserum, Biological and medical sciences, Caenorhabditis - genetics, Classical genetics, quantitative genetics, hybrids, CUTICLE, CUTICULA, CUTICULE, Epitopes, Epitopes - analysis, Epitopes - genetics, Fluorescent Antibody Technique, Fundamental and applied biological sciences. Psychology, GENE, GENES, Genetic Complementation Test, Genetic mutation, Genetics of eukaryotes. Biological and molecular evolution, Genotype, IMMUNOGENETICS, IMMUNOGENETIQUE, INMUNOGENETICA, Invertebrata, Medical genetics, MUTACION, MUTATION, NEMATODE, NEMATODE DES PLANTES, Nematode larvae, NEMATODES, NEMATODOS, NEMATODOS DE LAS PLANTAS, Phenotypes, PLANT NEMATODES, RADIOIODINATION, Recombination, Genetic, STRAINS, Surface antigens