Proceedings of the National Academy of Sciences - PNAS, 1990-01, Vol.87 (2), p.816-820
1990
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Details
- Autor(en) / Beteiligte
- Titel
- Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts
- Ist Teil von
- Proceedings of the National Academy of Sciences - PNAS, 1990-01, Vol.87 (2), p.816-820
- Ort / Verlag
- Washington, DC: National Academy of Sciences of the United States of America
- Erscheinungsjahr
- 1990
- Quelle
- Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
- Beschreibungen/Notizen
- We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0027-8424eISSN: 1091-6490DOI: 10.1073/pnas.87.2.816Titel-ID: cdi_proquest_miscellaneous_15773895
- Format
- –
- Schlagworte
- Base Sequence, Biological and medical sciences, Cells, Chimera, Chlorophylls, Cloning, Molecular, DNA - genetics, Flow Cytometry, FOTOSINTESIS, Fundamental and applied biological sciences. Psychology, GENE, Gene expression, Gene Expression - drug effects, Gene fusion, GENES, GENETIC TRANSFORMATION, Guard cells, HERBICIDAS, HERBICIDE, HERBICIDES, Herbicides - pharmacology, Mesophyll, Molecular and cellular biology, Molecular genetics, Molecular Sequence Data, Mosaic Viruses, Nicotiana - genetics, NICOTIANA TABACUM, NORFLURAZON, PHOTOSYNTHESE, PHOTOSYNTHESIS, Plants, Plants - genetics, Plants, Toxic, PLASMIDE, PLASMIDIOS, PLASMIDS, PROTOPLASTE, PROTOPLASTO, PROTOPLASTS, Protoplasts - metabolism, Pyridazines - pharmacology, Transcription, Genetic, Transfection, TRANSFORMACION GENETICA, TRANSFORMATION GENETIQUE