Details

Autor(en) / Beteiligte

• MUTLU, M
• TURHAN, N
• ERCAN, M. T
• ÖZDURAL, A. R
• CELEBI, S. S

Titel

Kinetics of invertase immobilised on poly(phe-lys) coated polystyrene beads

Ist Teil von

• Biotechnology techniques, 1996, Vol.10 (2), p.71-76

Ort / Verlag

London: Chapman and Hall

Erscheinungsjahr

1996

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• Surface of polystyrene beads was modified by poly(phe-lys) for invertase immobilization. The optimum immobilization conditions of invertase were: 0.01% (w/v) poly(phe-lys), 2% (v/v) glutaraldehyde at 25 degree C and pH 4.5. The kinetics of sucrose hydrolysis by free and immobilized invertase in a batch reactor at pH 4.5 and 55 degree C gave K sub(m) and V sub(max) values for sucrose with free and immobilized invertase of 81, 114 mM and 10.1, 9.2 mu mol glucose/min.mg enzyme, respectively. The deactivation rate constants of free and immobilized invertase were 0.0347 and 0.0098/min, respectively.