Tumor biology, 2014-08, Vol.35 (8), p.7743-7754
2014
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- Autor(en) / Beteiligte
- Titel
- Long noncoding RNA SPRY4-IT1 is upregulated in esophageal squamous cell carcinoma and associated with poor prognosis
- Ist Teil von
- Tumor biology, 2014-08, Vol.35 (8), p.7743-7754
- Ort / Verlag
- Dordrecht: Springer Netherlands
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- LncRNA SPRY4-IT1 has been shown to promote the progression of melanoma. However, the role of lncRNA SPRY4-IT1 in human esophageal squamous cell carcinoma (ESCC) remains unclear. The purpose of this study is to investigate the clinical significance and biological functions of SPRY4-IT1 in ESCC. The expression levels of lncRNA SPRY4-IT in 92 ESCC patients and 8 ESCC cell lines were evaluated by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The prognostic significance was evaluated using Kaplan–Meier and Cox regression analyses. Small interfering RNA (siRNA) was used to suppress SPRY4-IT1 expression in ESCC cell lines. Both in vitro and in vivo assays were performed to further explore its role in tumor progression. SPRY4-IT1 levels were significantly higher in ESCC tissues and cells than in corresponding adjacent noncancerous tissues and nontumorigenic esophageal epithelial cells, and the ESCC patients with higher SPRY4-IT1 expression had an advanced clinical stage and poorer prognosis than those with lower SPRY4-IT1 expression. The multivariate analysis revealed that SPRY4-IT1 expression level is an independent prognostic factor in ESCC patients. In vitro assays demonstrated that knockdown of SPRY4-IT1 reduced cell proliferation, invasiveness, and migration. In vivo assays demonstrated that knockdown of SPRY4-IT1 decreases cell growth. SPRY4-IT1 is a novel molecule involved in ESCC progression, which may provide a potential prognostic biomarker and a potential target for therapeutic intervention.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1010-4283eISSN: 1423-0380DOI: 10.1007/s13277-014-2013-yTitel-ID: cdi_proquest_miscellaneous_1566829007
- Format
- –
- Schlagworte
- Aged, Animals, Biomedical and Life Sciences, Biomedicine, Cancer Research, Carcinoma, Squamous Cell - genetics, Carcinoma, Squamous Cell - mortality, Cell adhesion & migration, Cell growth, Cell Line, Tumor, Cell Movement, Cell Proliferation, Esophageal cancer, Esophageal Neoplasms - genetics, Esophageal Neoplasms - mortality, Esophageal Squamous Cell Carcinoma, Female, Humans, Intracellular Signaling Peptides and Proteins - genetics, Male, Medical prognosis, Metastasis, Mice, Mice, Inbred BALB C, Middle Aged, Neoplasm Invasiveness, Nerve Tissue Proteins - genetics, Prognosis, Research Article, Ribonucleic acid, RNA, RNA, Long Noncoding - genetics, RNA, Long Noncoding - physiology, Up-Regulation