Details

Autor(en) / Beteiligte

• Shiono, Katsuhiro
• Ando, Miho
• Nishiuchi, Shunsaku
• Takahashi, Hirokazu
• Watanabe, Kohtaro
• Nakamura, Motoaki
• Matsuo, Yuichi
• Yasuno, Naoko
• Yamanouchi, Utako
• Fujimoto, Masaru
• Takanashi, Hideki
• Ranathunge, Kosala
• Franke, Rochus B
• Shitan, Nobukazu
• Nishizawa, Naoko K
• Takamure, Itsuro
• Yano, Masahiro
• Tsutsumi, Nobuhiro
• Schreiber, Lukas
• Yazaki, Kazufumi
• Nakazono, Mikio
• Kato, Kiyoaki

Titel

RCN1/OsABCG5, an ATP‐binding cassette (ABC) transporter, is required for hypodermal suberization of roots in rice (Oryza sativa)

Ist Teil von

• The Plant journal : for cell and molecular biology, 2014-10, Vol.80 (1), p.40-51

Ort / Verlag

England: Blackwell Scientific Publishers and BIOS Scientific Publishers in association with the Society for Experimental Biology

Erscheinungsjahr

2014

Link zum Volltext

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Suberin is a complex polymer composed of aliphatic and phenolic compounds. It is a constituent of apoplastic plant interfaces. In many plant species, including rice (Oryza sativa), the hypodermis in the outer part of roots forms a suberized cell wall (the Casparian strip and/or suberin lamellae), which inhibits the flow of water and ions and protects against pathogens. To date, there is no genetic evidence that suberin forms an apoplastic transport barrier in the hypodermis. We discovered that a rice reduced culm number1 (rcn1) mutant could not develop roots longer than 100 mm in waterlogged soil. The mutated gene encoded an ATP‐binding cassette (ABC) transporter named RCN1/OsABCG5. RCN1/OsABCG5 gene expression in the wild type was increased in most hypodermal and some endodermal roots cells under stagnant deoxygenated conditions. A GFP‐RCN1/OsABCG5 fusion protein localized at the plasma membrane of the wild type. Under stagnant deoxygenated conditions, well suberized hypodermis developed in wild types but not in rcn1 mutants. Under stagnant deoxygenated conditions, apoplastic tracers (periodic acid and berberine) were blocked at the hypodermis in the wild type but not in rcn1, indicating that the apoplastic barrier in the mutant was impaired. The amount of the major aliphatic suberin monomers originating from C₂₈ and C₃₀ fatty acids or ω‐OH fatty acids was much lower in rcn1 than in the wild type. These findings suggest that RCN1/OsABCG5 has a role in the suberization of the hypodermis of rice roots, which contributes to formation of the apoplastic barrier.