Details

Autor(en) / Beteiligte

• TODOROKI, Kenichiro
• NAKANO, Tatsuki
• WATANABE, Hiroki
• MIN, Jun Zhe
• INOUE, Koichi
• ISHIKAWA, Yoshinobu
• TOYO’OKA, Toshimasa

Titel

Computational Prediction of Diastereomeric Separation Behavior of Fluorescent o-Phthalaldehyde Derivatives of Amino Acids

Ist Teil von

• Analytical Sciences, 2014/09/10, Vol.30(9), pp.865-870

Ort / Verlag

Singapore: The Japan Society for Analytical Chemistry

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• We have developed a convenient method for predicting the LC resolution of amino acid diastereomers through computational calculations. For acquiring experimental data, we derivatized 10 amino acids using o-phthalaldehyde and N-acetyl-L-cysteine as fluorogenic diastereomer-forming reagents and analyzed the diastereomers using reversed-phase LC and fluorescence detection. For theoretical chemical calculations, we used the publicly available semi-empirical calculation software MOPAC2012. Using the obtained experimental and theoretical data, we determined the change in analytical resolution with differences in the structure of the diastereomers. From the results obtained, we concluded that greater conformational change through diastereomeric derivatization induced an increase in the contact area with the stationary phase, leading to higher resolution.