Details

Autor(en) / Beteiligte

• Braga, Cleiton Márcio Pinto
• Delabona, Priscila da Silva
• Lima, Deise Juliana da Silva
• Paixão, Douglas Antônio Alvaredo
• Pradella, José Geraldo da Cruz
• Farinas, Cristiane Sanchez

Titel

Addition of feruloyl esterase and xylanase produced on-site improves sugarcane bagasse hydrolysis

Ist Teil von

• Bioresource technology, 2014-10, Vol.170, p.316-324

Ort / Verlag

Kidlington: Elsevier Ltd

Erscheinungsjahr

2014

Quelle

MEDLINE

Beschreibungen/Notizen

• •Accessory enzymes produced on-site can improve biomass degradation.•Feruloyl esterase and xylanase improved sugarcane bagasse hydrolysis.•Sugarcane bagasse hydrolysis increased 36% with supplementation. Accessory enzymes that assist biomass degradation could be used to improve the recovery of fermentable sugar for use in biorefineries. In this study, different fungal strains isolated from the Amazon rainforest were evaluated in terms of their ability to produce feruloyl esterase (FAE) and xylanase enzymes, and an assessment was made of the contributions of the enzymes in the hydrolysis of pretreated sugarcane bagasse. In the selection step, screening using plate assays was followed by shake flask submerged cultivations. After carbon source selection and cultivation in a stirred-tank bioreactor, Aspergillusoryzae P21C3 proved to be a promising strain for production of the enzymes. Supplementation of a commercial enzyme preparation with 30% (v/v) crude enzymatic complex from A. oryzae P21C3 increased the conversion of cellulose derived from pretreated sugarcane bagasse by 36%. Supplementation with FAE and xylanase enzymes produced on-site can therefore be used to improve the hydrolysis of sugarcane bagasse.