Details

Autor(en) / Beteiligte

• Chon, Jung-Whan
• Kim, Young-Ji
• Kim, Hong-Seok
• Kim, Dong-Hyeon
• Kim, Hyunsook
• Song, Kwang-Young
• Seo, Kun-Ho

Titel

Supplementation of Bolton broth with triclosan improves detection of Campylobacter jejuni and Campylobacter coli in chicken carcass rinse

Ist Teil von

• International journal of food microbiology, 2014-07, Vol.181, p.37-39

Ort / Verlag

Amsterdam: Elsevier B.V

Erscheinungsjahr

2014

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We compared Bolton enrichment broth supplemented with antimicrobial triclosan (T-Bolton broth) and normal Bolton broth for the isolation of Campylobacter jejuni (C. jejuni) and Campylobacter coli (C. coli) from chicken carcass rinse. Whole chickens were rinsed with buffered peptone water prior to enrichment in normal Bolton broth or T-Bolton broth, followed by inoculation onto modified charcoal-cefoperazone-deoxycholate agar (mCCDA). Suspect colonies were confirmed by PCR. We observed a significantly higher number of C. jejuni or C. coli-positive samples in the T-Bolton broth (71.3%) than in the normal Bolton broth (27.5%) (p<0.05). Furthermore, the number of contaminated mCCDA plates was lower after enrichment in T-Bolton broth (3.8%) than in the normal Bolton broth (75%) (p<0.05), indicating that T-Bolton broth has higher selectivity. Finally, we identified extended-spectrum β-lactamase-producing Escherichia coli as the predominant competing flora in normal Bolton broth. In conclusion, the use of T-Bolton broth results in significant elimination of competing bacteria. •A new Campylobacter selective broth broth (T-Bolton) was developed.•T-Bolton broth to isolate Campylobacter from chicken carcass rinse was evaluated.•T-Bolton broth yielded better (p<0.05) isolation rate and selectivity than normal Bolton broth.