Details

Autor(en) / Beteiligte

• García-Almodóvar, R. C
• Petri, C
• Padilla, I. M. G
• Burgos, L

Titel

Combination of site-specific recombination and a conditional selective marker gene allows for the production of marker-free tobacco plants

Ist Teil von

• Plant cell, tissue and organ culture, 2014-02, Vol.116 (2), p.205-215

Ort / Verlag

Dordrecht: Springer-Verlag

Erscheinungsjahr

2014

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• We have designed an innovative construct (pX6-DAO1) combining the chemical inducible Cre-LoxP system and the conditional selectable marker gene dao1 to obtain marker-free transgenic tobacco plants. Nicotiana tabaccum transgenic lines were regenerated on medium with 6 mM D-alanine. The DNA site-specific recombination was controlled by the inducer ß-estradiol. Regeneration on medium containing 5 μM ß-estradiol and 8 mM D-valine was not obtained. However, leaf disks from all transgenic lines regenerated in the presence of ß-estradiol, although only 9.4 % of regenerated buds developed solid marker-free shoots. Partial recombination was found in 71.7 % of buds, and no recombination was detected in only 18.9 % of buds. Nevertheless, when leaf disks from chimeric shoots were cultured in medium with 8 mM D-valine, only marker-free buds regenerated, and no partial recombinants were detected. Similarly, marker-free plants were produced from T₂ seeds, obtained from chimeric ß-estradiol-induced T₁ plants, with 100 % efficiency in selective D-valine medium.