Details

Autor(en) / Beteiligte

• Anunciacao, C.E
• Guimaraes, W.V
• Araujo, E.F
• Muchovej, R.M.C
• Souza, D.R.N. e

Titel

Production and regeneration of protoplasts of Pisolithus tinctorius

Ist Teil von

• Biotechnology techniques, 1990-01, Vol.4 (3), p.215-220

Ort / Verlag

London: Chapman and Hall

Erscheinungsjahr

1990

Quelle

SpringerLINK Contemporary (Konsortium Baden-Württemberg)

Beschreibungen/Notizen

• The production of protoplasts of the ectomycorrhizal fungus Pisolithus tinctorius , isolate Pt 571, was improved using young mycelium treated with Novozym-234 dissolved in 0.5 M mannitol in the proportion 20:1:0.15 (mg mycelium: mg enzyme:ml mannitol) and incubated at 25 degree C for 4 hours. Plating the protoplasts on the surface of solid medium containing 0.5 M mannitol increased the regeneration rate.