Details

Autor(en) / Beteiligte

• Gonzalez-Muñoz, Elena
• Arboleda-Estudillo, Yohanna
• Otu, Hasan H
• Cibelli, Jose B

Titel

Cell reprogramming. Histone chaperone ASF1A is required for maintenance of pluripotency and cellular reprogramming

Ist Teil von

• Science (American Association for the Advancement of Science), 2014-08, Vol.345 (6198), p.822-825

Ort / Verlag

United States

Erscheinungsjahr

2014

Quelle

American Association for the Advancement of Science

Beschreibungen/Notizen

• Unfertilized oocytes have the intrinsic capacity to remodel sperm and the nuclei of somatic cells. The discoveries that cells can change their phenotype from differentiated to embryonic state using oocytes or specific transcription factors have been recognized as two major breakthroughs in the biomedical field. Here, we show that ASF1A, a histone-remodeling chaperone specifically enriched in the metaphase II human oocyte, is necessary for reprogramming of human adult dermal fibroblasts (hADFs) into undifferentiated induced pluripotent stem cell. We also show that overexpression of just ASF1A and OCT4 in hADFs exposed to the oocyte-specific paracrine growth factor GDF9 can reprogram hADFs into pluripotent cells. Our Report underscores the importance of studying the unfertilized MII oocyte as a means to understand the molecular pathways governing somatic cell reprogramming.