Details

Autor(en) / Beteiligte

• Crawford, M.S
• Wang, W.Y
• Jensen, K.G

Titel

Identification of the primary lesion in a protoporphyrin accumulating mutant of Chlamydomonas reinhardtii

Ist Teil von

• Molecular & general genetics, 1982-01, Vol.188 (1), p.1-6

Erscheinungsjahr

1982

Beschreibungen/Notizen

• A group of chlorophyll deficient mutants (br sub(s) mutants) of Chlamydomonas) accumulates protoporphyrin and has poorly developed chloroplast membrane systems. In order to determine whether a poorly developed chloroplast membrane system is the reason for or the result of, the inability of the br sub(s) mutants to metabolize protoporphyrin to chlorophyll, a second mutation was selected which restored chlorophyll synthesis in br sub(s) mutants. One such double mutant (br sub(s)-2 g-4) was analyzed. It is concluded that a defect in chloroplast membrane development resulting from the br sub(s)-2 mutation causes an apparent defect in magnesium chelation by protoprophyrin. This is consistant with evidence that chlorophyll biosynthesis from magnesium protoporphyrin to chlorophyll takes place on the chloroplast membranes.