Developmental cell, 2014-01, Vol.28 (2), p.189-202
2014
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Details
- Autor(en) / Beteiligte
- Titel
- The Hox Gene Abd-B Controls Stem Cell Niche Function in the Drosophila Testis
- Ist Teil von
- Developmental cell, 2014-01, Vol.28 (2), p.189-202
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2014
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Proper niche architecture is critical for stem cell function, yet only few upstream regulators are known. Here, we report that the Hox transcription factor Abdominal-B (Abd-B), active in premeiotic spermatocytes of Drosophila testes, is essential for positioning the niche to the testis anterior by regulating integrin in neighboring somatic cyst cells. Abd-B also non-cell-autonomously controls critical features within the niche, including centrosome orientation and division rates of germline stem cells. By using genome-wide binding studies, we find that Abd-B mediates its effects on integrin localization by directly controlling at multiple levels the signaling activity of the Sev ligand Boss via its direct targets src42A and sec63, two genes involved in protein trafficking and recycling. Our data show that Abd-B, through local signaling between adjucent cell types, provides positional cues for integrin localization, which is critical for placement of the distant stem cell niche and stem cell activity. •Abd-B indirectly controls stem cell activity in the Drosophila larval testis•Integrin is the mediator for Abd-B-dependent stem cell niche positioning•Integrin localization is non-cell-autonomously controlled via the Boss/Sev pathway•Abd-B regulates Boss signaling activity via its direct targets src42A and sec63 Papagiannouli et al. show that Hox transcription factor Abd-B controls stem cell activity in the Drosophila larval testis via non-cell-autonomous regulation of integrin localization. Abd-B binds to promoters of src42A and sec63, trafficking components required for Boss/Sev pathway activity, which in turn controls integrin localization in neighboring cells.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1534-5807eISSN: 1878-1551DOI: 10.1016/j.devcel.2013.12.016Titel-ID: cdi_proquest_miscellaneous_1551642441
- Format
- –
- Schlagworte
- Adult Stem Cells - cytology, Adult Stem Cells - metabolism, Adult Stem Cells - physiology, Animals, Cell Division, Centrosome - metabolism, Drosophila, Drosophila - cytology, Drosophila - genetics, Drosophila - growth & development, Drosophila - metabolism, Drosophila Proteins - genetics, Drosophila Proteins - metabolism, Eye Proteins - genetics, Eye Proteins - metabolism, Genome, Insect, Homeodomain Proteins - genetics, Homeodomain Proteins - metabolism, Integrins - genetics, Integrins - metabolism, Male, Membrane Glycoproteins - genetics, Membrane Glycoproteins - metabolism, Protein Binding, Proto-Oncogene Proteins pp60(c-src) - genetics, Proto-Oncogene Proteins pp60(c-src) - metabolism, Receptor Protein-Tyrosine Kinases - genetics, Receptor Protein-Tyrosine Kinases - metabolism, Receptors, Peptide - genetics, Receptors, Peptide - metabolism, Spermatocytes - cytology, Spermatocytes - metabolism, Spermatocytes - physiology, Stem Cell Niche, Testis - cytology, Testis - growth & development, Testis - metabolism