The Journal of immunology (1950), 2013-03, Vol.190 (5), p.2362-2371
2013
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Details
- Autor(en) / Beteiligte
- Titel
- FcαRI-mediated inhibition of IL-12 production and priming by IFN-γ of human monocytes and dendritic cells
- Ist Teil von
- The Journal of immunology (1950), 2013-03, Vol.190 (5), p.2362-2371
- Ort / Verlag
- United States
- Erscheinungsjahr
- 2013
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We showed that IgA induces IL-10 in monocytes and dendritic cells. Because reciprocal inhibition exists between IL-10 and IL-12, we explored whether IgA could regulate this other immunoregulatory cytokine. In human monocytes and monocyte-derived dendritic cells preincubated with IFN-γ before stimulation by LPS, suppression of p40 and IL-12p70 production was observed upon IgA treatment during IFN-γ priming. Washout experiments and inhibition of IFN-γ-induced CXCL10 (IP-10) and FcγRI (CD64) indicated that inhibition by IgA occurred at both the LPS and IFN-γ levels. Inhibition was not affected by blockade of IL-10 or MAPK but involved FcαRI/CD89-mediated suppression of STAT1 phosphorylation. These data indicate that FcαRI ligation on human monocytes and dendritic cells inhibits IL-12 expression and type 1 activation by interfering with STAT1 activation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0022-1767eISSN: 1550-6606DOI: 10.4049/jimmunol.1201128Titel-ID: cdi_proquest_miscellaneous_1551630755
- Format
- –
- Schlagworte
- Antigens, CD - genetics, Antigens, CD - immunology, Cells, Cultured, Chemokine CXCL10 - antagonists & inhibitors, Chemokine CXCL10 - genetics, Chemokine CXCL10 - immunology, Dendritic Cells - cytology, Dendritic Cells - drug effects, Dendritic Cells - immunology, Flow Cytometry, Gene Expression Regulation - drug effects, Humans, I-kappa B Proteins - genetics, I-kappa B Proteins - immunology, Immunoglobulin A - immunology, Immunoglobulin A - pharmacology, Interferon-gamma - immunology, Interferon-gamma - pharmacology, Interleukin-10 - antagonists & inhibitors, Interleukin-10 - genetics, Interleukin-10 - immunology, Interleukin-12 - antagonists & inhibitors, Interleukin-12 - biosynthesis, Interleukin-12 - genetics, Interleukin-12 - immunology, Lipopolysaccharides - pharmacology, Monocytes - cytology, Monocytes - drug effects, Monocytes - immunology, NF-KappaB Inhibitor alpha, Phosphorylation - drug effects, Receptors, Fc - genetics, Receptors, Fc - immunology, Receptors, IgG - genetics, Receptors, IgG - immunology, Signal Transduction - drug effects, STAT1 Transcription Factor - genetics, STAT1 Transcription Factor - immunology