Details

Autor(en) / Beteiligte

• Marwitz, Sebastian
• Kolarova, Julia
• Reck, Martin
• Reinmuth, Niels
• Kugler, Christian
• Schädlich, Ines
• Haake, Andrea
• Zabel, Peter
• Vollmer, Ekkehard
• Siebert, Reiner
• Goldmann, Torsten
• Ammerpohl, Ole

Titel

The tissue is the issue: improved methylome analysis from paraffin-embedded tissues by application of the HOPE technique

Ist Teil von

• Laboratory investigation, 2014-08, Vol.94 (8), p.927-933

Ort / Verlag

New York: Nature Publishing Group US

Erscheinungsjahr

2014

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Alterations in the DNA methylome are characteristic for numerous diseases and a typical hallmark of cancer. Therefore, DNA methylation is currently under investigation in research labs and has also entered diagnostics. Recently, protocols like the BeadChip technology have become commercially available to study DNA methylation in an array format and semiquantitative fashion. However, it is known that fixation of the sample material with formalin prior to BeadChip analysis can affect the results. In this study we compared the influence of fixation on the outcome of BeadChip analysis. From six patients each a lung cancer tissue sample and a corresponding tumor-free lung tissue sample were collected. The samples were separated into three pieces. One piece of each sample was fixed with formalin, another one by the non-cross-linking HOPE technique (Hepes-glutamic acid buffer mediated Organic solvent Protection Effect). Subsequently, both became paraffin embedded. As a reference, the remaining third piece was cryopreserved. In addition we used three adenocarcinoma cell lines (H838, A549, and H1650) to validate the results from patient tissues. We show that using the HOPE technique instead of formalin largely prevents the introduction of formalin-fixation related artifacts. An ANOVA analysis significantly separated HOPE- and cryopreserved from formalin-fixed samples (FDR<0.05), while differences in the methylation data obtained from HOPE-fixed and cryopreserved material were minor. Consequently, HOPE fixation is superior to formalin fixation if a subsequent BeadChip analysis of paraffin-embedded sample material is intended.