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Antioxidant Capacity of Ugni molinae Fruit Extract on Human Erythrocytes: An In Vitro Study
Ist Teil von
The Journal of membrane biology, 2014-08, Vol.247 (8), p.703-712
Ort / Verlag
New York: Springer US
Erscheinungsjahr
2014
Quelle
MEDLINE
Beschreibungen/Notizen
Ugni
molinae
is an important source of molecules with strong antioxidant activity widely used as a medicinal plant in Southern Chile–Argentina. Total phenol concentration from its fruit extract was 10.64 ± 0.04 mM gallic acid equivalents. Analysis by means of HPLC/MS indicated the presence of the anthocyanins cyanidin and peonidin, and the flavonol quercitin, all in glycosylated forms. Its antioxidant properties were assessed in human erythrocytes in vitro exposed to HClO oxidative stress. Scanning electron microscopy showed that HClO induced an alteration in erythrocytes from a normal shape to echinocytes; however, this change was highly attenuated in samples containing
U. molinae
extracts. It also had a tendency in order to reduce the hemolytic effect of HClO. In addition, X-ray diffraction experiments were performed in dimyristoylphosphatidylcholine (DMPC) and dimyristoylphosphatidylethanolamine bilayers, classes of lipids preferentially located in the outer and inner monolayers, respectively, of the human erythrocyte membrane. It was observed that
U. molinae
only interacted with DMPC. Results by fluorescence spectroscopy on DMPC large unilamellar vesicles and isolated unsealed human erythrocyte membranes also showed that it interacted with the erythrocyte membrane and DMPC. It is possible that the location of
U. molinae
components into the membrane outer monolayer might hinder the diffusion of HClO and of free radicals into cell membranes and the consequent decrease of the kinetics of free radical reactions.