Plant, cell and environment, 2014-09, Vol.37 (9), p.2036-2050
2014
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Details
- Autor(en) / Beteiligte
- Titel
- Role of H₂O₂ dynamics in brassinosteroid‐induced stomatal closure and opening in Solanum lycopersicum
- Ist Teil von
- Plant, cell and environment, 2014-09, Vol.37 (9), p.2036-2050
- Ort / Verlag
- Oxford: Blackwell Science
- Erscheinungsjahr
- 2014
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Brassinosteroids (BRs) are essential for plant growth and development; however, their roles in the regulation of stomatal opening or closure remain obscure. Here, the mechanism underlying BR‐induced stomatal movements is studied. The effects of 24‐epibrassinolide (EBR) on the stomatal apertures of tomato (Solanum lycopersicum) were measured by light microscopy using epidermal strips of wild type (WT), the abscisic acid (ABA)‐deficient notabilis (not) mutant, and plants silenced for SlBRI1, SlRBOH1 and SlGSH1. EBR induced stomatal opening within an appropriate range of concentrations, whereas high concentrations of EBR induced stomatal closure. EBR‐induced stomatal movements were closely related to dynamic changes in H₂O₂ and redox status in guard cells. The stomata of SlRBOH1‐silenced plants showed a significant loss of sensitivity to EBR. However, ABA deficiency abolished EBR‐induced stomatal closure but did not affect EBR‐induced stomatal opening. Silencing of SlGSH1, the critical gene involved in glutathione biosynthesis, disrupted glutathione redox homeostasis and abolished EBR‐induced stomatal opening. The results suggest that transient H₂O₂ production is essential for poising the cellular redox status of glutathione, which plays an important role in BR‐induced stomatal opening. However, a prolonged increase in H₂O₂ facilitated ABA signalling and stomatal closure.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0140-7791eISSN: 1365-3040DOI: 10.1111/pce.12275Titel-ID: cdi_proquest_miscellaneous_1549184187
- Format
- –
- Schlagworte
- abscisic acid, Abscisic Acid - pharmacology, Biological and medical sciences, biosynthesis, brassinosteroids, Brassinosteroids - pharmacology, Fundamental and applied biological sciences. Psychology, Gene Silencing - drug effects, genes, glutathione, Glutathione - pharmacology, growth and development, guard cells, homeostasis, hydrogen peroxide, Hydrogen Peroxide - metabolism, light microscopy, Lycopersicon esculentum - drug effects, Lycopersicon esculentum - enzymology, Lycopersicon esculentum - physiology, Models, Biological, Movement - drug effects, mutants, NADPH oxidase, NADPH Oxidases - metabolism, Oxidation-Reduction - drug effects, photosynthesis, plant growth, Plant Proteins - genetics, Plant Proteins - metabolism, Plant Stomata - cytology, Plant Stomata - drug effects, Plant Stomata - enzymology, Plant Stomata - physiology, Solanum lycopersicum, Steroids, Heterocyclic - pharmacology, stomata, stomatal movement, tomatoes