Details

Autor(en) / Beteiligte

• McClintock, Timothy S.
• Schütte, Klemens
• Ache, Barry W.

Titel

Failure to implicate cAMP in transduction in lobster olfactory receptor cells

Ist Teil von

• Chemical senses, 1989-12, Vol.14 (6), p.817-827

Ort / Verlag

Oxford: Oxford University Press

Erscheinungsjahr

1989

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• The possible role of adenosine 3',5'-cyclic monophosphate (cAMP) in olfactory transduction in the spiny lobster was investigated using radioimmunoassay of cAMP and intracellular recording. Application of forskolin or 1-isobutyl-3-methylxanthine increased cAMP levels in intact sensilla containing the chemoreceptive outer dendritic segments of the lobster olfactory receptor cell, thereby demonstrating adenylate cyclase and phosphodiesterase activity in the sensilla. A complex odor mixture and identified excitatory odor molecules failed to stimulate the production of cAMP, however In intracellular recordings, superfusion of the outer dendritic segments with forskolin, 1-isobutyl-3-methylxanthine and cyclic nucleotide analogs had no direct effect on odor-responsive cells. These compounds did cause infrequent enhancements (six of 42 cells) of odor-evoked receptor potentials, but processes other than transduction are the most likely causes of this effect. We conclude that cAMP-dependent transduction mechanisms are unlikely to mediate most odor responses in lobsters, in contrast to transduction mechanisms in amphibians and rats.